Real-time Measurement of Cellular Refractive Index and Thickness Using Cell Culture Chip

نویسندگان

  • J. J. Gu
  • Y. F. Yu
  • E. P. Li
  • S. H. Ng
  • P. H. Yap
  • X. Q. Zhou
  • T. H. Cheng
  • A. Q. Liu
چکیده

This paper reports the measurement of refractive index (RI) and thickness of living cells using cell culture chip integrated with micro-indicators via immersion refractometry. In the experiment, the RI and the thickness of the cultured MadinDarby canine kidney (MDCK) cell line are obtained simultaneously. As this method features high accuracy, no damage to a group of the living cells and real time measurement during long-term cell culture, it is promising for many applications such as detecting pathological changes of cells and cancer cell detection.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Modeling of measurement error in refractive index determination of fuel cell using neural network and genetic algorithm

Abstract: In this paper, a method for determination of refractive index in membrane of fuel cell on basis of three-longitudinal-mode laser heterodyne interferometer is presented. The optical path difference between the target and reference paths is fixed and phase shift is then calculated in terms of refractive index shift. The measurement accuracy of this system is limited by nonlinearity erro...

متن کامل

Refractive Index Measurement of Single Living Cell Using a Biophotonic Chip for Cancer Diagnosis Applications

This paper presents a new approach to determining the effective refractive index of single living cell. By means of it, the refractive index of living cell can be automatically measured in real time without any extra treatments on the cell, meanwhile, offering low cost and high accuracy of disease diagnosis.

متن کامل

Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy.

We have developed a digital holographic microscope (DHM), in a transmission mode, adapted to the quantitative study of cellular dynamics. Living cells in culture are optically probed by measuring the phase shift they produce on the transmitted wave front. The high temporal stability of the phase signal, equivalent to lambda/1800, and the low acquisition time (~20micros) enable to monitor cellul...

متن کامل

Tip sensor probe for changing refractive index measurement in small volumes

In this paper, a tapered tip optical fiber probe sensor for localized refractive index (RI) measurements is presented. This sensor’s interaction with analytes is confined to a few micro-meters which makes it a promising candidate for in-vivo or even intra-cellular RI monitoring. This tapered tip was simply fabricated by etching optical fiber with hydrofluoric acid to a conic shape with a sub-m...

متن کامل

Optical-mechanical properties of diseased cells measured by interferometry

Interferometric phase microscopy (IPM) enables to obtain quantitative optical thickness profiles of transparent samples, including live cells in-vitro, and track them in time with sub-nanometer accuracy without any external labeling, contact or force application on the sample. The optical thickness measured by IPM is a multiplication between the cell integral refractive index differences and it...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2008